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- | ==== Biological Samples and DNA ===== | ||
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- | === Biological Samples === | ||
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- | Biological samples were collected for the first time in 1999; | ||
- | non-fasting blood samples were used to measure indicators of risk | ||
- | factors for cardiovascular disease (cholesterol, | ||
- | triglyceride and glycoslated haemoglobin) and nutritional markers (red | ||
- | cell folate, plasma folate, plasma vitamin B12 and plasma ferritin). | ||
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- | The samples also include a blood sample and a cheek swab from which | ||
- | DNA was derived. | ||
- | cell line cultures. | ||
- | techniques using the BD vacutainer system. | ||
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- | Stored biological samples available from 1999 comprise of EDTA plasma, | ||
- | lithium heparin plasma, cell lines, and DNA. The following tables list the current data available from these samples. | ||
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- | ^ Table 1 - Measurements taken in NSHD at 60-64 years ^^ | ||
- | |Cardiovascular assessment | ||
- | |Musculoskeletal assessment | ||
- | |Tests of functional capacity, biological function, and anthropometric measures|Verbal memory, search speed and concentration, | ||
- | |Assessment of free-living physical activity | ||
- | |Cardio-respiratory fitness | ||
- | |Overnight fasting 50ml blood sample | ||
- | |Overnight fasting urine sample | ||
- | |Salivary samples | ||
- | |Scales or questions | ||
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- | ^ Table 2 - Biomarkers available at age 53 and 63. Biomarkers highlighted in bold are not present in other UK birth cohorts | ||
- | ^System | ||
- | |Metabolic | ||
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- | |Diet & Iron Markers| | ||
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- | |Inflammatory | ||
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- | |Neuro-Endocrine | ||
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- | | ::: |Sex hormone binding globulin, SHBG (nmol/ | ||
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- | |Kidney & Liver | ||
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- | ==== DNA ==== | ||
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- | Around 2,900 study members have given consent for DNA to be extracted | ||
- | from blood and/or buccal samples and LHA was given ethical approval in | ||
- | 2007 to use these samples for genetic studies of lifelong health and | ||
- | ageing. Genetic studies help us to elucidate biological mechanisms of | ||
- | ageing and understand why people with similar life experiences age | ||
- | differently. | ||
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- | The NSHD DNA Repository supports high calibre studies of human health | ||
- | and disease that make the best use of the genetic resource in | ||
- | conjunction with the existing life course data. Dr Andrew Wong | ||
- | coordinates the genetic research of the NSHD and is responsible for | ||
- | the safe storage and efficient use of the samples, which are managed | ||
- | by Source BioScience. | ||
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- | Further information on the access to the NSHD DNA Repository is | ||
- | available {{mrepo: | ||