mrepo:topics:dna

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mrepo:topics:dna [16/05/2018 @ 14:14] adammooremrepo:topics:dna [02/09/2022 @ 16:14] (current) nmpo
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-[[:wiki:Home|{{:MRC-LHA-header-graphic.png}}]] 
 ==== DNA ===== ==== DNA =====
 ---- ----
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 coordinates the genetic research of the NSHD and is responsible for coordinates the genetic research of the NSHD and is responsible for
 the safe storage and efficient use of the samples, which are managed the safe storage and efficient use of the samples, which are managed
-by Source BioScience.+by LGC. 
 +\\ \\ 
  
-{{mrepo:Guidance-NSHD_biological-samples.pdf|Further information}} on the access to the NSHD DNA Repository is +In summary, DNA samples have been collected at three time points: 
-available.+ 
 +__[[questionnaires:1999|1999]]__  
 +  * Buccal (N=2918) and blood (2717) 
 +  * Extraction using Gentra Puregene kit (Swallow) 
 +\\  
 + 
 +__[[questionnaires:2009|2006-10]]__  
 +  * Blood (1777) 
 +  * Extraction using Autopure DNA extraction robot (MRC Epi) 
 +\\  
 +  
 +__[[questionnaires:2015|2014-16]]__  
 +  * Blood (1912) 
 +  * Extraction using Autopure DNA extraction robot (MRC Epi) 
 +\\  
 + 
 +Genotyping has been conducted primarily using the blood DNA from age 53 years, this includes single SNP typing, SNPlex and Sequenom, cardiometabochip, the new Illumina DrugDev consortium array and the updated NeuroX2 array.  The buccal DNA has since been quantified (GenProbe in 2013) and will be used for future genotyping.   
 + 
 +\\  
 + 
 +{{mrepo:Guidance-NSHD_biological-samples.pdf|Further information}} on access to the NSHD DNA Repository is available.
  
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