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- | ==== Biological Samples and DNA ===== | + | ==== DNA ===== |
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- | === Biological Samples === | ||
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- | Biological samples were collected for the first time in [[questionnaires: | ||
- | non-fasting blood samples were used to measure indicators of risk | ||
- | factors for cardiovascular disease (cholesterol, | ||
- | triglyceride and glycoslated haemoglobin) and nutritional markers (red | ||
- | cell folate, plasma folate, plasma vitamin B12 and plasma ferritin). | ||
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- | The samples also include a blood sample and a cheek swab from which | ||
- | DNA was derived. | ||
- | cell line cultures. | ||
- | techniques using the BD vacutainer system. | ||
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- | Stored biological samples available from [[questionnaires: | ||
- | lithium heparin plasma, cell lines, and DNA. The following tables list the current data available from these samples. | ||
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- | ^ Table 1 - Measurements taken in NSHD at 60-64 years ^^ | ||
- | |**Cardiovascular assessment** | ||
- | |**Musculoskeletal assessment** | ||
- | |**Tests of functional capacity, biological function and anthropometric measures**|Verbal memory, search speed and concentration, | ||
- | |**Assessment of free-living physical activity** | ||
- | |**Cardio-respiratory fitness** | ||
- | |**Overnight fasting 50ml blood sample** | ||
- | |**Overnight fasting urine sample** | ||
- | |**Salivary samples** | ||
- | |**Scales or questions** | ||
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- | \\ | ||
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- | ^ Table 2 - Biomarkers available at age 53 and 63. Biomarkers highlighted in bold are not present in other UK birth cohorts | ||
- | ^System | ||
- | |**Metabolic** | ||
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- | |**Diet & Iron Markers**| | ||
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- | |**Inflammatory** | ||
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- | |**Neuro-Endocrine** | ||
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- | | ::: |Sex hormone binding globulin, SHBG (nmol/ | ||
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- | |**Kidney & Liver** | ||
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- | ---- | ||
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- | === DNA === | ||
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Around 2,900 study members have given consent for DNA to be extracted | Around 2,900 study members have given consent for DNA to be extracted | ||
from blood and / or buccal samples and LHA was given ethical approval in | from blood and / or buccal samples and LHA was given ethical approval in | ||
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coordinates the genetic research of the NSHD and is responsible for | coordinates the genetic research of the NSHD and is responsible for | ||
the safe storage and efficient use of the samples, which are managed | the safe storage and efficient use of the samples, which are managed | ||
- | by Source BioScience. | + | by LGC. |
+ | \\ \\ | ||
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+ | In summary, DNA samples have been collected at three time points: | ||
+ | |||
+ | __[[questionnaires: | ||
+ | * Buccal (N=2918) and blood (2717) | ||
+ | * Extraction using Gentra Puregene kit (Swallow) | ||
+ | \\ | ||
+ | |||
+ | __[[questionnaires: | ||
+ | * Blood (1777) | ||
+ | * Extraction using Autopure DNA extraction robot (MRC Epi) | ||
+ | \\ | ||
+ | |||
+ | __[[questionnaires: | ||
+ | * Blood (1912) | ||
+ | * Extraction using Autopure DNA extraction robot (MRC Epi) | ||
+ | \\ | ||
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+ | Genotyping has been conducted primarily using the blood DNA from age 53 years, this includes single SNP typing, SNPlex and Sequenom, cardiometabochip the new Illumina DrugDev consortium array and the updated NeuroX2 array. | ||
+ | |||
+ | \\ | ||
{{mrepo: | {{mrepo: | ||
available. | available. | ||