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mrepo:topics:dnaandbiologicalsamples [12/03/2018 @ 15:53] – [Biological Samples and DNA] adammoore | mrepo:topics:dnaandbiologicalsamples [14/05/2018 @ 11:14] – adammoore | ||
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==== Biological Samples and DNA ===== | ==== Biological Samples and DNA ===== | ||
- | ==== Biological Samples | + | ---- |
+ | === Biological Samples === | ||
- | Biological samples were collected for the first time in 1999; | + | Biological samples were collected for the first time in [[questionnaires: |
non-fasting blood samples were used to measure indicators of risk | non-fasting blood samples were used to measure indicators of risk | ||
factors for cardiovascular disease (cholesterol, | factors for cardiovascular disease (cholesterol, | ||
triglyceride and glycoslated haemoglobin) and nutritional markers (red | triglyceride and glycoslated haemoglobin) and nutritional markers (red | ||
cell folate, plasma folate, plasma vitamin B12 and plasma ferritin). | cell folate, plasma folate, plasma vitamin B12 and plasma ferritin). | ||
+ | |||
The samples also include a blood sample and a cheek swab from which | The samples also include a blood sample and a cheek swab from which | ||
DNA was derived. | DNA was derived. | ||
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techniques using the BD vacutainer system. | techniques using the BD vacutainer system. | ||
- | Stored biological samples available from 1999 comprise of EDTA plasma, | + | Stored biological samples available from [[questionnaires: |
lithium heparin plasma, cell lines, and DNA. The following tables list the current data available from these samples. | lithium heparin plasma, cell lines, and DNA. The following tables list the current data available from these samples. | ||
^ Table 1 - Measurements taken in NSHD at 60-64 years ^^ | ^ Table 1 - Measurements taken in NSHD at 60-64 years ^^ | ||
- | |Cardiovascular assessment | + | |**Cardiovascular assessment** |Vascular structure and function: carotid intima-medial thickness (IMT) and arterial distensibility (GE Vivid-I), carotid/ |
- | |Musculoskeletal assessment | + | |**Musculoskeletal assessment** |DXA (QDR 4500 Discovery): Hip (total, femoral neck, trochanter, Ward’s), lumbar spine (L1-4), whole body & region BMD, fat & lean mass, vertebral fracture assessment, aortic calcification score\\ \\ pQCT (Stratec XCT 2000) radius: 4% site(trabecular, |
- | |Tests of functional capacity, biological function, and anthropometric measures|Verbal memory, search speed and concentration, | + | |**Tests of functional capacity, biological function and anthropometric measures**|Verbal memory, search speed and concentration, |
- | |Assessment of free-living physical activity | + | |**Assessment of free-living physical activity** |Five continuous days by combined heart rate and movement sensor (Actiheart) with individual calibration. |
- | |Cardio-respiratory fitness | + | |**Cardio-respiratory fitness** |
- | |Overnight fasting 50ml blood sample | + | |**Overnight fasting 50ml blood sample** |//See table 2 for details of sample observations// |
- | |Overnight fasting urine sample | + | |**Overnight fasting urine sample** |
- | |Salivary samples | + | |**Salivary samples** |One salivary sample was collected at the visit and a further three in the following 24 hours (evening, next day waking, and 30 minutes later). |
- | |Scales or questions | + | |**Scales or questions** |Rose angina, intermittent claudication, |
+ | \\ | ||
^ Table 2 - Biomarkers available at age 53 and 63. Biomarkers highlighted in bold are not present in other UK birth cohorts | ^ Table 2 - Biomarkers available at age 53 and 63. Biomarkers highlighted in bold are not present in other UK birth cohorts | ||
^System | ^System | ||
- | |Metabolic | + | |**Metabolic** |
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- | |Diet & Iron Markers| | + | |**Diet & Iron Markers**| |
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- | |Inflammatory | + | |**Inflammatory** | |** Anti-Thyroperoxidase, |
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- | |Neuro-Endocrine | + | |**Neuro-Endocrine** |
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| ::: |Sex hormone binding globulin, SHBG (nmol/ | | ::: |Sex hormone binding globulin, SHBG (nmol/ | ||
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- | |Kidney & Liver | + | |**Kidney & Liver** | |
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+ | ---- | ||
- | + | === DNA === | |
- | ==== DNA ==== | + | |
Around 2,900 study members have given consent for DNA to be extracted | Around 2,900 study members have given consent for DNA to be extracted | ||
- | from blood and/or buccal samples and LHA was given ethical approval in | + | from blood and / or buccal samples and LHA was given ethical approval in |
2007 to use these samples for genetic studies of lifelong health and | 2007 to use these samples for genetic studies of lifelong health and | ||
- | ageing. Genetic studies help us to elucidate biological mechanisms of | + | ageing. |
+ | Genetic studies help us to elucidate biological mechanisms of | ||
ageing and understand why people with similar life experiences age | ageing and understand why people with similar life experiences age | ||
differently. | differently. | ||
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by Source BioScience. | by Source BioScience. | ||
- | Further information on the access to the NSHD DNA Repository is | + | {{mrepo: |
- | available | + | available. |