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==== DNA ===== | ==== DNA ===== | ||
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- | Biological samples were collected for the first time in [[questionnaires: | ||
- | non-fasting blood samples were used to measure indicators of risk | ||
- | factors for cardiovascular disease (cholesterol, | ||
- | triglyceride and glycoslated haemoglobin) and nutritional markers (red | ||
- | cell folate, plasma folate, plasma vitamin B12 and plasma ferritin). | ||
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- | The samples also include a blood sample and a cheek swab from which | ||
- | DNA was derived. | ||
- | cell line cultures. | ||
- | techniques using the BD vacutainer system. | ||
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- | Stored biological samples available from [[questionnaires: | ||
- | lithium heparin plasma, cell lines, and DNA. The following tables list the current data available from these samples. | ||
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- | ^ Table 1 - Measurements taken in NSHD at 60-64 years ^^ | ||
- | |**Cardiovascular assessment** | ||
- | |**Musculoskeletal assessment** | ||
- | |**Tests of functional capacity, biological function and anthropometric measures**|Verbal memory, search speed and concentration, | ||
- | |**Assessment of free-living physical activity** | ||
- | |**Cardio-respiratory fitness** | ||
- | |**Overnight fasting 50ml blood sample** | ||
- | |**Overnight fasting urine sample** | ||
- | |**Salivary samples** | ||
- | |**Scales or questions** | ||
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- | ^ Table 2 - Biomarkers available at age 53 and 63. Biomarkers highlighted in bold are not present in other UK birth cohorts | ||
- | ^System | ||
- | |**Metabolic** | ||
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- | |**Diet & Iron Markers**| | ||
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- | |**Inflammatory** | ||
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- | |**Neuro-Endocrine** | ||
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- | | ::: |Sex hormone binding globulin, SHBG (nmol/ | ||
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- | |**Kidney & Liver** | ||
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- | ---- | ||
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- | === DNA === | ||
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Around 2,900 study members have given consent for DNA to be extracted | Around 2,900 study members have given consent for DNA to be extracted | ||
from blood and / or buccal samples and LHA was given ethical approval in | from blood and / or buccal samples and LHA was given ethical approval in | ||
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coordinates the genetic research of the NSHD and is responsible for | coordinates the genetic research of the NSHD and is responsible for | ||
the safe storage and efficient use of the samples, which are managed | the safe storage and efficient use of the samples, which are managed | ||
- | by Source BioScience. | + | by LGC. |
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+ | In summary, DNA samples have been collected at three time points: | ||
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+ | __[[questionnaires: | ||
+ | * Buccal (N=2918) and blood (2717) | ||
+ | * Extraction using Gentra Puregene kit (Swallow) | ||
+ | \\ | ||
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+ | __[[questionnaires: | ||
+ | * Blood (1777) | ||
+ | * Extraction using Autopure DNA extraction robot (MRC Epi) | ||
+ | \\ | ||
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+ | __[[questionnaires: | ||
+ | * Blood (1912) | ||
+ | * Extraction using Autopure DNA extraction robot (MRC Epi) | ||
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+ | Genotyping has been conducted primarily using the blood DNA from age 53 years, this includes single SNP typing, SNPlex and Sequenom, cardiometabochip, | ||
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+ | \\ | ||
- | {{mrepo: | + | {{mrepo: |
- | available. | + | |