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mrepo:topics:biologicalsamples [24/09/2018 @ 16:49]
nmpo
mrepo:topics:biologicalsamples [03/10/2018 @ 16:42] (current)
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Line 162: Line 162:
  
 DNA extractions were conducted on the EDTA tube used in the first round blood haematology analysis. Samples for lymphoid cell line transformations were originally collected in 1999; for those that did not previously provide a sample, one was collected in 2006-10. \\ \\  DNA extractions were conducted on the EDTA tube used in the first round blood haematology analysis. Samples for lymphoid cell line transformations were originally collected in 1999; for those that did not previously provide a sample, one was collected in 2006-10. \\ \\ 
-{{mrepo:bloods_0610.jpg?​600|Bloods collected in 2006-10}}+ 
 +<​HTML>​ 
 +<table x:str=""​ style="​border-collapse:​ collapse; background-color:​ rgb(255, 255, 255); width: 900px;"​ border="​2"​ cellpadding="​2"​ cellspacing="​2">​ 
 +<​tbody>​ 
 +<tr style="​height:​ 12.75pt;" height="​17">​ 
 +<td class="​xl22"​ style="​text-align: center; width: 100px;"​ x:num> 
 +<​p><​b>​Order of collection priority</​b></​p>​ 
 +</​td>​ 
 +<td class="​xl22"​ style="​text-align:​ center; width: 300px;"​ x:num> 
 +<​p><​b>​Blood Tube</​b></​p>​ 
 +</​td>​ 
 +<td class="​xl22"​ style="​text-align:​ center; width: 300px;"​ x:num> 
 +<​p><​b>​Aliquots for first round blood analysis</​b></​p>​ 
 +</​td>​ 
 +<td class="​xl22"​ style="​text-align:​ center; width: 200px;"​ x:num> 
 +<​p><​b>​Repository aliquots</​b></​p>​ 
 +</​td>​ 
 +</​tr>​ 
 +<​tr>​ 
 +<td > 
 +<​p>​1</​p>​ 
 +</​td>​ 
 +<td > 
 +<p>1 x 2.7ml EDTA tube (EN1)</​p>​ 
 +</​td>​ 
 +<​td>​ 
 +<​p>​None as sample was sent for haematology analysis</​p>​ 
 +</​td>​ 
 +<td > 
 +<​p>​EN1 generated for DNA extraction</​p>​ 
 +</​td>​ 
 +</​tr>​ 
 + 
 +<​tr>​ 
 +<td > 
 +<​p>​2</​p>​ 
 +</​td>​ 
 +<td > 
 +<p>1 x 2.7ml EDTA tube (EN2)</​p>​ 
 +</​td>​ 
 +<​td>​ 
 +<p> None as sample was sent for HbA1c and red cell folate analysis <p> 
 +</​td>​ 
 +<td > 
 +<​p>​None</​p>​ 
 +</​td>​ 
 +</​tr>​ 
 + 
 +<​tr>​ 
 +<td > 
 +<​p>​3</​p>​ 
 +</​td>​ 
 +<td > 
 +<p>1 x 2.7ml Fluoride tube (FL N1)</​p>​ 
 +</​td>​ 
 +<​td>​ 
 +<​p>​FLA1 for glucose analysis</​p>​ 
 +</​td>​ 
 +<td > 
 +<​p>​None</​p>​ 
 +</​td>​ 
 +</​tr>​ 
 + 
 +<​tr>​ 
 +<td > 
 +<​p>​4*</​p>​ 
 +</​td>​ 
 +<td > 
 +<p>1 x 7.5ml Lithium heparin tube (trace metal) (LH N1)</​p>​ 
 +</​td>​ 
 +<​td>​ 
 +<p>LH INS for insulin; LH VITC for vitamin C analysis</​p>​ 
 +</​td>​ 
 +<td > 
 +<p>LH B1, LH C1, LH C2, LH C3 </​p>​ 
 +</​td>​ 
 +</​tr>​ 
 + 
 +<tr > 
 +<td > 
 +<​p>​5</​p>​ 
 +</​td>​ 
 +<td > 
 +<p>1 x 8.5ml CPDA (for cell lines if required) (CP N1)</​p>​ 
 +</​td>​ 
 +<​td>​ 
 +<​p>​None as sent to ECACC for cell lines</​p>​ 
 +</​td>​ 
 +<td > 
 +<​p>​None </​p>​ 
 +</​td>​ 
 +</​tr>​ 
 + 
 +<tr > 
 +<td > 
 +<​p>​6</​p>​ 
 +</​td>​ 
 +<td > 
 +<p>1 x 7.5ml Serum tube (SE N1)</​p>​ 
 +</​td>​ 
 +<​td>​ 
 +<p> Samples from the two serum tubes were used to produce priority aliquots SE A1 for TFTs and SE A2 for analyses at NHR</​p>​ 
 +</​td>​ 
 +<td > 
 +<p>SE B1, SE C1, SE C2</​p>​ 
 +</​td>​ 
 +</​tr>​ 
 + 
 +<tr > 
 +<td > 
 +<​p>​7*</​p>​ 
 +</​td>​ 
 +<td > 
 +<p>1 x 7.5ml Serum tube (SE N2)</​p>​ 
 +</​td>​ 
 +<​td>​ 
 +<p> Samples from the two serum tubes were used to produce priority aliquots SE A1 for TFTs and SE A2 for analyses at NHR</​p>​ 
 +</​td>​ 
 +<td > 
 +<p>SE B1, SE C1, SE C2</​p>​ 
 +</​td>​ 
 +</​tr>​ 
 + 
 +<tr > 
 +<td > 
 +<​p>​8*</​p>​ 
 +</​td>​ 
 +<td > 
 +<p>1 x 4.9ml EDTA tube (E N3)</​p>​ 
 +</​td>​ 
 +<​td>​ 
 +<​p>​None</​p>​ 
 +</​td>​ 
 +<td > 
 +<p>E B1, E C1</​p>​ 
 +</​td>​ 
 +</​tr>​ 
 +<tr > 
 +<td > 
 +<​p>​8*</​p>​ 
 +</​td>​ 
 +<td > 
 +<p>1 x 10ml Citrate tube (CI N1)</​p>​ 
 +</​td>​ 
 +<​td>​ 
 +<​p>​None</​p>​ 
 +</​td>​ 
 +<td > 
 +<p>CI B1, CI C1, CI C2</​p>​ 
 +</​td>​ 
 +</​tr>​ 
 + 
 +</​tbody>​ 
 +</​table>​ 
 +</​HTML>​ 
 +*Blood tubes/​aliquots not collected at remote home visits 
 + 
 + 
 + 
 + 
 + 
 \\ \\  \\ \\ 
 __Urine__ __Urine__
Line 179: Line 340:
    
 A number of assays have been undertaken and the remaining aliquots of EDTA, citrate plasma and serum are stored at the Bristol Bioresource Laboratories. ​ DNA extractions have been conducted at MRC Epidemiology,​ Cambridge, as per the 2006-10 samples. ​ \\ \\  A number of assays have been undertaken and the remaining aliquots of EDTA, citrate plasma and serum are stored at the Bristol Bioresource Laboratories. ​ DNA extractions have been conducted at MRC Epidemiology,​ Cambridge, as per the 2006-10 samples. ​ \\ \\ 
-{{mrepo:bloods_14.jpg?​600|Bloods collected in 2014-16}}+ 
 +<​HTML>​ 
 +<table x:str=""​ style="​border-collapse:​ collapse; background-color:​ rgb(255, 255, 255); width: 900px;"​ border="​2"​ cellpadding="​2"​ cellspacing="​2">​ 
 +<​tbody>​ 
 +<tr style="​height:​ 12.75pt;" height="​17">​ 
 +<td class="​xl22"​ style="​text-align:​ center; width: 100px;"​ x:num> 
 +<​p><​b>​Order of collection priority</​b></​p>​ 
 +</​td>​ 
 +<td class="​xl22"​ style="​text-align:​ center; width: 300px;"​ x:num> 
 +<​p><​b>​Blood Tube</​b></​p>​ 
 +</​td>​ 
 +<td class="​xl22"​ style="​text-align:​ center; width: 300px;"​ x:num> 
 +<​p><​b>​Aliquots for first round blood analysis</​b></​p>​ 
 +</​td>​ 
 +<td class="​xl22"​ style="​text-align:​ center; width: 200px;"​ x:num> 
 +<​p><​b>​Repository aliquots</​b></​p>​ 
 +</​td>​ 
 +</​tr>​ 
 +<​tr>​ 
 +<td > 
 +<​p>​1</​p>​ 
 +</​td>​ 
 +<td > 
 +<p>1 x 2.7ml citrate</​p>​ 
 +</​td>​ 
 +<​td>​ 
 +<​p>​None </​p>​ 
 +</​td>​ 
 +<td > 
 +<​p>​CIT101 – CIT104</​p>​ 
 +</​td>​ 
 +</​tr>​ 
 + 
 +<​tr>​ 
 +<td > 
 +<​p>​2</​p>​ 
 +</​td>​ 
 +<td > 
 +<p>1 x 5ml Serum</​p>​ 
 +</​td>​ 
 +<​td>​ 
 +<p> Cholesterol,​ HDL-, LDL-cholesterol,​ triglycerides<​p>​ 
 +</​td>​ 
 +<td > 
 +<​p>​SEA101 – SEA107</​p>​ 
 +</​td>​ 
 +</​tr>​ 
 + 
 +<​tr>​ 
 +<td > 
 +<​p>​3</​p>​ 
 +</​td>​ 
 +<td > 
 +<p>1 x 5ml Serum</​p>​ 
 +</​td>​ 
 +<​td>​ 
 +<​p>​None</​p>​ 
 +</​td>​ 
 +<td > 
 +<​p>​SEA201 – SEA207</​p>​ 
 +</​td>​ 
 +</​tr>​ 
 + 
 +<​tr>​ 
 +<td > 
 +<​p>​4</​p>​ 
 +</​td>​ 
 +<td > 
 +<p>1 x 5ml Serum</​p>​ 
 +</​td>​ 
 +<​td>​ 
 +<​p>​None</​p>​ 
 +</​td>​ 
 +<td > 
 +<​p>​SEA301 – SEA307 </​p>​ 
 +</​td>​ 
 +</​tr>​ 
 + 
 +<tr > 
 +<td > 
 +<​p>​5</​p>​ 
 +</​td>​ 
 +<td > 
 +<p>1 x 4ml EDTA</​p>​ 
 +</​td>​ 
 +<​td>​ 
 +<​p>​HbA1c,​ haemoglobin,​ FBC, WCC</​p>​ 
 +</​td>​ 
 +<td > 
 +<​p>​DNA </​p>​ 
 +</​td>​ 
 +</​tr>​ 
 + 
 +<tr > 
 +<td > 
 +<​p>​6</​p>​ 
 +</​td>​ 
 +<td > 
 +<p>1 x 4ml EDTA </​p>​ 
 +</​td>​ 
 +<​td>​ 
 +<p> None</​p>​ 
 +</​td>​ 
 +<td > 
 +<​p>​EN2_01 – EN2_05</​p>​ 
 +</​td>​ 
 +</​tr>​ 
 + 
 +</​tbody>​ 
 +</​table>​ 
 +</​HTML>​ 
 + 
 + 
 ---- ----
 \\  \\