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mrepo:topics:biologicalsamples [16/05/2018 @ 13:32]
adammoore
mrepo:topics:biologicalsamples [03/10/2018 @ 16:42] (current)
nmpo
Line 17: Line 17:
 Stored biological samples available from [[questionnaires:​1999|1999]] comprise of EDTA plasma, Stored biological samples available from [[questionnaires:​1999|1999]] comprise of EDTA plasma,
 lithium heparin plasma, cell lines, and DNA.  The following tables list the current data available from these samples.\\ \\  lithium heparin plasma, cell lines, and DNA.  The following tables list the current data available from these samples.\\ \\ 
-{{mrepo:bloods_1999.jpg?​600|Bloods collected in 1999}}+<​HTML>​ 
 +<table x:str=""​ style="​border-collapse:​ collapse; background-color:​ rgb(255, 255, 255); width: 900px;"​ border="​2"​ cellpadding="​2"​ cellspacing="​2">​ 
 +<​tbody>​ 
 +<tr style="​height:​ 12.75pt;" height="​17">​ 
 +<td class="​xl22"​ style="​text-align:​ center; width: 100px;"​ x:num> 
 +<​p><​b>​Order of Collection</​b></​p>​ 
 +</​td>​ 
 +<td class="​xl22"​ style="​text-align:​ center; width: 100px;"​ x:num> 
 +<​p><​b>​Blood Tube</​b></​p>​ 
 +</​td>​ 
 +<td class="​xl22"​ style="​text-align:​ center; width: 300px;"​ x:num> 
 +<​p><​b>​Analytes (destination)</​b></​p>​ 
 +</​td>​ 
 +<td class="​xl22"​ style="​text-align:​ center; width: 200px;"​ x:num> 
 +<​p><​b>​Repository aliquots</​b></​p>​ 
 +</​td>​ 
 +<td class="​xl22"​ style="​text-align:​ center; width: 200px;"​ x:num> 
 +<​p><​b>​Comment</​b></​p>​ 
 +</​td>​ 
 +</​tr>​ 
 +<​tr>​ 
 +<td > 
 +<​p>​1</​p>​ 
 +</​td>​ 
 +<td > 
 +<​p>​Buccal swabs</​p>​ 
 +</​td>​ 
 +<​td>​ 
 +<​p>​DNA (MRC-HBGU)</​p>​ 
 +</​td>​ 
 +<td > 
 +<​p>​DNA</​p>​ 
 +</​td>​ 
 +<td > 
 +<​p>​DNA quantified by Genprobe</​p>​ 
 +</​td>​ 
 +</​tr>​ 
 + 
 +<​tr>​ 
 +<td > 
 +<​p>​2</​p>​ 
 +</​td>​ 
 +<td > 
 +<p>1 x 3ml EDTA tube</​p>​ 
 +</​td>​ 
 +<​td>​ 
 +<​p>​DNA (MRC-HBGU)</​p>​ 
 +</​td>​ 
 +<td > 
 +<​p>​DNA</​p>​ 
 +</​td>​ 
 +<td > 
 +<​p>​Limited availability</​p>​ 
 +</​td>​ 
 +</​tr>​ 
 + 
 +<​tr>​ 
 +<td > 
 +<​p>​3</​p>​ 
 +</​td>​ 
 +<td > 
 +<p>1 x 2ml EDTA tube</​p>​ 
 +</​td>​ 
 +<​td>​ 
 +<​p>​DNA (MRC-HBGU)</​p>​ 
 +</​td>​ 
 +<td > 
 +<​p>​DNA</​p>​ 
 +</​td>​ 
 +<td > 
 +<​p>​Limited availability</​p>​ 
 +</​td>​ 
 +</​tr>​ 
 + 
 +<​tr>​ 
 +<td > 
 +<​p>​4</​p>​ 
 +</​td>​ 
 +<td > 
 +<p>1 x 6ml ACD tube</​p>​ 
 +</​td>​ 
 +<​td>​ 
 +<​p>​ECACC</​p>​ 
 +</​td>​ 
 +<td > 
 +<p> ECACC </​p>​ 
 +</​td>​ 
 +<td > 
 +<​p>​EBV transformed lymphoid cell lines created</​p>​ 
 +</​td>​ 
 +</​tr>​ 
 + 
 +<tr > 
 +<td > 
 +<​p>​5</​p>​ 
 +</​td>​ 
 +<td > 
 +<p>1 x 4ml Lithium heparin tube</​p>​ 
 +</​td>​ 
 +<​td>​ 
 +<​p>​Cholesterol,​ HDL cholesterol,​ triglyceride (RVI) </​p>​ 
 +</​td>​ 
 +<td > 
 +<​p>​Lithium heparin </​p>​ 
 +</​td>​ 
 +<td > 
 +<​p>​Limited availability </​p>​ 
 +</​td>​ 
 +</​tr>​ 
 + 
 +<tr > 
 +<td > 
 +<​p>​6</​p>​ 
 +</​td>​ 
 +<td > 
 +<p>1 x 3ml EDTA tube</​p>​ 
 +</​td>​ 
 +<​td>​ 
 +<​p>​Red cell folate, plasma folate, plasma B12, plasma ferritin (RVI)</​p>​ 
 +</​td>​ 
 +<td > 
 +<​p>​EDTA plasma</​p>​ 
 +</​td>​ 
 +<td > 
 +<​p>​Limited availability </​p>​ 
 +</​td>​ 
 +</​tr>​ 
 +</​tbody>​ 
 +</​table>​ 
 +</​HTML>​ 
 + 
 + 
 + 
 + 
 ---- ----
 \\  \\ 
Line 28: Line 162:
  
 DNA extractions were conducted on the EDTA tube used in the first round blood haematology analysis. Samples for lymphoid cell line transformations were originally collected in 1999; for those that did not previously provide a sample, one was collected in 2006-10. \\ \\  DNA extractions were conducted on the EDTA tube used in the first round blood haematology analysis. Samples for lymphoid cell line transformations were originally collected in 1999; for those that did not previously provide a sample, one was collected in 2006-10. \\ \\ 
-{{mrepo:bloods_0610.jpg?​600|Bloods collected in 2006-10}}+ 
 +<​HTML>​ 
 +<table x:str=""​ style="​border-collapse:​ collapse; background-color:​ rgb(255, 255, 255); width: 900px;"​ border="​2"​ cellpadding="​2"​ cellspacing="​2">​ 
 +<​tbody>​ 
 +<tr style="​height:​ 12.75pt;" height="​17">​ 
 +<td class="​xl22"​ style="​text-align: center; width: 100px;"​ x:num> 
 +<​p><​b>​Order of collection priority</​b></​p>​ 
 +</​td>​ 
 +<td class="​xl22"​ style="​text-align:​ center; width: 300px;"​ x:num> 
 +<​p><​b>​Blood Tube</​b></​p>​ 
 +</​td>​ 
 +<td class="​xl22"​ style="​text-align:​ center; width: 300px;"​ x:num> 
 +<​p><​b>​Aliquots for first round blood analysis</​b></​p>​ 
 +</​td>​ 
 +<td class="​xl22"​ style="​text-align:​ center; width: 200px;"​ x:num> 
 +<​p><​b>​Repository aliquots</​b></​p>​ 
 +</​td>​ 
 +</​tr>​ 
 +<​tr>​ 
 +<td > 
 +<​p>​1</​p>​ 
 +</​td>​ 
 +<td > 
 +<p>1 x 2.7ml EDTA tube (EN1)</​p>​ 
 +</​td>​ 
 +<​td>​ 
 +<​p>​None as sample was sent for haematology analysis</​p>​ 
 +</​td>​ 
 +<td > 
 +<​p>​EN1 generated for DNA extraction</​p>​ 
 +</​td>​ 
 +</​tr>​ 
 + 
 +<​tr>​ 
 +<td > 
 +<​p>​2</​p>​ 
 +</​td>​ 
 +<td > 
 +<p>1 x 2.7ml EDTA tube (EN2)</​p>​ 
 +</​td>​ 
 +<​td>​ 
 +<p> None as sample was sent for HbA1c and red cell folate analysis <p> 
 +</​td>​ 
 +<td > 
 +<​p>​None</​p>​ 
 +</​td>​ 
 +</​tr>​ 
 + 
 +<​tr>​ 
 +<td > 
 +<​p>​3</​p>​ 
 +</​td>​ 
 +<td > 
 +<p>1 x 2.7ml Fluoride tube (FL N1)</​p>​ 
 +</​td>​ 
 +<​td>​ 
 +<​p>​FLA1 for glucose analysis</​p>​ 
 +</​td>​ 
 +<td > 
 +<​p>​None</​p>​ 
 +</​td>​ 
 +</​tr>​ 
 + 
 +<​tr>​ 
 +<td > 
 +<​p>​4*</​p>​ 
 +</​td>​ 
 +<td > 
 +<p>1 x 7.5ml Lithium heparin tube (trace metal) (LH N1)</​p>​ 
 +</​td>​ 
 +<​td>​ 
 +<p>LH INS for insulin; LH VITC for vitamin C analysis</​p>​ 
 +</​td>​ 
 +<td > 
 +<p>LH B1, LH C1, LH C2, LH C3 </​p>​ 
 +</​td>​ 
 +</​tr>​ 
 + 
 +<tr > 
 +<td > 
 +<​p>​5</​p>​ 
 +</​td>​ 
 +<td > 
 +<p>1 x 8.5ml CPDA (for cell lines if required) (CP N1)</​p>​ 
 +</​td>​ 
 +<​td>​ 
 +<​p>​None as sent to ECACC for cell lines</​p>​ 
 +</​td>​ 
 +<td > 
 +<​p>​None </​p>​ 
 +</​td>​ 
 +</​tr>​ 
 + 
 +<tr > 
 +<td > 
 +<​p>​6</​p>​ 
 +</​td>​ 
 +<td > 
 +<p>1 x 7.5ml Serum tube (SE N1)</​p>​ 
 +</​td>​ 
 +<​td>​ 
 +<p> Samples from the two serum tubes were used to produce priority aliquots SE A1 for TFTs and SE A2 for analyses at NHR</​p>​ 
 +</​td>​ 
 +<td > 
 +<p>SE B1, SE C1, SE C2</​p>​ 
 +</​td>​ 
 +</​tr>​ 
 + 
 +<tr > 
 +<td > 
 +<​p>​7*</​p>​ 
 +</​td>​ 
 +<td > 
 +<p>1 x 7.5ml Serum tube (SE N2)</​p>​ 
 +</​td>​ 
 +<​td>​ 
 +<p> Samples from the two serum tubes were used to produce priority aliquots SE A1 for TFTs and SE A2 for analyses at NHR</​p>​ 
 +</​td>​ 
 +<td > 
 +<p>SE B1, SE C1, SE C2</​p>​ 
 +</​td>​ 
 +</​tr>​ 
 + 
 +<tr > 
 +<td > 
 +<​p>​8*</​p>​ 
 +</​td>​ 
 +<td > 
 +<p>1 x 4.9ml EDTA tube (E N3)</​p>​ 
 +</​td>​ 
 +<​td>​ 
 +<​p>​None</​p>​ 
 +</​td>​ 
 +<td > 
 +<p>E B1, E C1</​p>​ 
 +</​td>​ 
 +</​tr>​ 
 +<tr > 
 +<td > 
 +<​p>​8*</​p>​ 
 +</​td>​ 
 +<td > 
 +<p>1 x 10ml Citrate tube (CI N1)</​p>​ 
 +</​td>​ 
 +<​td>​ 
 +<​p>​None</​p>​ 
 +</​td>​ 
 +<td > 
 +<p>CI B1, CI C1, CI C2</​p>​ 
 +</​td>​ 
 +</​tr>​ 
 + 
 +</​tbody>​ 
 +</​table>​ 
 +</​HTML>​ 
 +*Blood tubes/​aliquots not collected at remote home visits 
 + 
 + 
 + 
 + 
 + 
 \\ \\  \\ \\ 
 __Urine__ __Urine__
Line 45: Line 340:
    
 A number of assays have been undertaken and the remaining aliquots of EDTA, citrate plasma and serum are stored at the Bristol Bioresource Laboratories. ​ DNA extractions have been conducted at MRC Epidemiology,​ Cambridge, as per the 2006-10 samples. ​ \\ \\  A number of assays have been undertaken and the remaining aliquots of EDTA, citrate plasma and serum are stored at the Bristol Bioresource Laboratories. ​ DNA extractions have been conducted at MRC Epidemiology,​ Cambridge, as per the 2006-10 samples. ​ \\ \\ 
-{{mrepo:bloods_14.jpg?​600|Bloods collected in 2014-16}}+ 
 +<​HTML>​ 
 +<table x:str=""​ style="​border-collapse:​ collapse; background-color:​ rgb(255, 255, 255); width: 900px;"​ border="​2"​ cellpadding="​2"​ cellspacing="​2">​ 
 +<​tbody>​ 
 +<tr style="​height:​ 12.75pt;" height="​17">​ 
 +<td class="​xl22"​ style="​text-align:​ center; width: 100px;"​ x:num> 
 +<​p><​b>​Order of collection priority</​b></​p>​ 
 +</​td>​ 
 +<td class="​xl22"​ style="​text-align:​ center; width: 300px;"​ x:num> 
 +<​p><​b>​Blood Tube</​b></​p>​ 
 +</​td>​ 
 +<td class="​xl22"​ style="​text-align:​ center; width: 300px;"​ x:num> 
 +<​p><​b>​Aliquots for first round blood analysis</​b></​p>​ 
 +</​td>​ 
 +<td class="​xl22"​ style="​text-align:​ center; width: 200px;"​ x:num> 
 +<​p><​b>​Repository aliquots</​b></​p>​ 
 +</​td>​ 
 +</​tr>​ 
 +<​tr>​ 
 +<td > 
 +<​p>​1</​p>​ 
 +</​td>​ 
 +<td > 
 +<p>1 x 2.7ml citrate</​p>​ 
 +</​td>​ 
 +<​td>​ 
 +<​p>​None </​p>​ 
 +</​td>​ 
 +<td > 
 +<​p>​CIT101 – CIT104</​p>​ 
 +</​td>​ 
 +</​tr>​ 
 + 
 +<​tr>​ 
 +<td > 
 +<​p>​2</​p>​ 
 +</​td>​ 
 +<td > 
 +<p>1 x 5ml Serum</​p>​ 
 +</​td>​ 
 +<​td>​ 
 +<p> Cholesterol,​ HDL-, LDL-cholesterol,​ triglycerides<​p>​ 
 +</​td>​ 
 +<td > 
 +<​p>​SEA101 – SEA107</​p>​ 
 +</​td>​ 
 +</​tr>​ 
 + 
 +<​tr>​ 
 +<td > 
 +<​p>​3</​p>​ 
 +</​td>​ 
 +<td > 
 +<p>1 x 5ml Serum</​p>​ 
 +</​td>​ 
 +<​td>​ 
 +<​p>​None</​p>​ 
 +</​td>​ 
 +<td > 
 +<​p>​SEA201 – SEA207</​p>​ 
 +</​td>​ 
 +</​tr>​ 
 + 
 +<​tr>​ 
 +<td > 
 +<​p>​4</​p>​ 
 +</​td>​ 
 +<td > 
 +<p>1 x 5ml Serum</​p>​ 
 +</​td>​ 
 +<​td>​ 
 +<​p>​None</​p>​ 
 +</​td>​ 
 +<td > 
 +<​p>​SEA301 – SEA307 </​p>​ 
 +</​td>​ 
 +</​tr>​ 
 + 
 +<tr > 
 +<td > 
 +<​p>​5</​p>​ 
 +</​td>​ 
 +<td > 
 +<p>1 x 4ml EDTA</​p>​ 
 +</​td>​ 
 +<​td>​ 
 +<​p>​HbA1c,​ haemoglobin,​ FBC, WCC</​p>​ 
 +</​td>​ 
 +<td > 
 +<​p>​DNA </​p>​ 
 +</​td>​ 
 +</​tr>​ 
 + 
 +<tr > 
 +<td > 
 +<​p>​6</​p>​ 
 +</​td>​ 
 +<td > 
 +<p>1 x 4ml EDTA </​p>​ 
 +</​td>​ 
 +<​td>​ 
 +<p> None</​p>​ 
 +</​td>​ 
 +<td > 
 +<​p>​EN2_01 – EN2_05</​p>​ 
 +</​td>​ 
 +</​tr>​ 
 + 
 +</​tbody>​ 
 +</​table>​ 
 +</​HTML>​ 
 + 
 + 
 ---- ----
 \\  \\