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mrepo:topics:biologicalsamples [16/05/2018 @ 11:13] – adammoore | mrepo:topics:biologicalsamples [25/09/2018 @ 15:52] – nmpo | ||
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Stored biological samples available from [[questionnaires: | Stored biological samples available from [[questionnaires: | ||
lithium heparin plasma, cell lines, and DNA. The following tables list the current data available from these samples.\\ \\ | lithium heparin plasma, cell lines, and DNA. The following tables list the current data available from these samples.\\ \\ | ||
- | {{mrepo:bloods_1999.jpg? | + | < |
+ | <table x:str="" | ||
+ | < | ||
+ | <tr style=" | ||
+ | <td class=" | ||
+ | < | ||
+ | </ | ||
+ | <td class=" | ||
+ | < | ||
+ | </ | ||
+ | <td class=" | ||
+ | < | ||
+ | </ | ||
+ | <td class=" | ||
+ | < | ||
+ | </ | ||
+ | <td class=" | ||
+ | < | ||
+ | </ | ||
+ | </ | ||
+ | < | ||
+ | <td > | ||
+ | < | ||
+ | </ | ||
+ | <td > | ||
+ | < | ||
+ | </ | ||
+ | < | ||
+ | < | ||
+ | </ | ||
+ | <td > | ||
+ | < | ||
+ | </ | ||
+ | <td > | ||
+ | < | ||
+ | </ | ||
+ | </ | ||
+ | |||
+ | < | ||
+ | <td > | ||
+ | < | ||
+ | </ | ||
+ | <td > | ||
+ | <p>1 x 3ml EDTA tube</ | ||
+ | </ | ||
+ | < | ||
+ | < | ||
+ | </ | ||
+ | <td > | ||
+ | < | ||
+ | </ | ||
+ | <td > | ||
+ | < | ||
+ | </ | ||
+ | </ | ||
+ | |||
+ | < | ||
+ | <td > | ||
+ | < | ||
+ | </ | ||
+ | <td > | ||
+ | <p>1 x 2ml EDTA tube</ | ||
+ | </ | ||
+ | < | ||
+ | < | ||
+ | </ | ||
+ | <td > | ||
+ | < | ||
+ | </ | ||
+ | <td > | ||
+ | < | ||
+ | </ | ||
+ | </ | ||
+ | |||
+ | < | ||
+ | <td > | ||
+ | < | ||
+ | </ | ||
+ | <td > | ||
+ | <p>1 x 6ml ACD tube</ | ||
+ | </ | ||
+ | < | ||
+ | < | ||
+ | </ | ||
+ | <td > | ||
+ | <p> ECACC </ | ||
+ | </ | ||
+ | <td > | ||
+ | < | ||
+ | </ | ||
+ | </ | ||
+ | |||
+ | <tr > | ||
+ | <td > | ||
+ | < | ||
+ | </ | ||
+ | <td > | ||
+ | <p>1 x 4ml Lithium heparin tube</ | ||
+ | </ | ||
+ | < | ||
+ | < | ||
+ | </ | ||
+ | <td > | ||
+ | < | ||
+ | </ | ||
+ | <td > | ||
+ | < | ||
+ | </ | ||
+ | </ | ||
+ | |||
+ | <tr > | ||
+ | <td > | ||
+ | < | ||
+ | </ | ||
+ | <td > | ||
+ | <p>1 x 3ml EDTA tube</ | ||
+ | </ | ||
+ | < | ||
+ | < | ||
+ | </ | ||
+ | <td > | ||
+ | < | ||
+ | </ | ||
+ | <td > | ||
+ | < | ||
+ | </ | ||
+ | </ | ||
+ | </ | ||
+ | </ | ||
+ | </ | ||
+ | |||
+ | |||
+ | |||
+ | |||
---- | ---- | ||
\\ | \\ | ||
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DNA extractions were conducted on the EDTA tube used in the first round blood haematology analysis. Samples for lymphoid cell line transformations were originally collected in 1999; for those that did not previously provide a sample, one was collected in 2006-10. \\ \\ | DNA extractions were conducted on the EDTA tube used in the first round blood haematology analysis. Samples for lymphoid cell line transformations were originally collected in 1999; for those that did not previously provide a sample, one was collected in 2006-10. \\ \\ | ||
- | {{mrepo: | ||
- | \\ \\ | ||
- | __Urine__ | ||
- | Overnight urine was collected, however the protocol for processing and storing the samples was changed during the data collection, resulting in a different number of aliquots being collected compared to the later period. The original protocol was inappropriate for albumin measurement as they should have been centrifuged; and they were stored at -20ºC instead | + | < |
- | \\ \\ | + | <table x: |
- | __Saliva__ | + | < |
+ | <tr style=" | ||
+ | <td class=" | ||
+ | < | ||
+ | </ | ||
+ | <td class=" | ||
+ | < | ||
+ | </ | ||
+ | <td class=" | ||
+ | < | ||
+ | </ | ||
+ | <td class=" | ||
+ | < | ||
+ | </ | ||
+ | </ | ||
+ | < | ||
+ | <td > | ||
+ | < | ||
+ | </ | ||
+ | <td > | ||
+ | <p>1 x 2.7ml EDTA tube (EN1)</ | ||
+ | </ | ||
+ | < | ||
+ | < | ||
+ | </ | ||
+ | <td > | ||
+ | < | ||
+ | </td> | ||
+ | </tr> | ||
- | The collection of saliva samples only began after a pilot study, and the protocol consisted of one sample collected on the day of the clinical assessment and a further three at home. HNR received all of these first samples, but for about 68 people | + | < |
+ | <td > | ||
+ | < | ||
+ | </ | ||
+ | <td > | ||
+ | <p>1 x 2.7ml EDTA tube (EN2)</ | ||
+ | </ | ||
+ | < | ||
+ | <p> None as sample was sent for HbA1c and red cell folate | ||
+ | </ | ||
+ | <td > | ||
+ | < | ||
+ | </ | ||
+ | </tr> | ||
- | ---- | + | <tr> |
- | \\ | + | <td > |
- | === Age 68-70=== | + | < |
+ | </ | ||
+ | <td > | ||
+ | <p>1 x 2.7 Fluoride tube (FL N1)</ | ||
+ | </ | ||
+ | < | ||
+ | < | ||
+ | </ | ||
+ | <td > | ||
+ | < | ||
+ | </ | ||
+ | </tr> | ||
- | \\ | + | < |
+ | <td > | ||
+ | < | ||
+ | </ | ||
+ | <td > | ||
+ | <p>1 x 7.5ml Lithium heparin tube (trace metal) (LH N1)</ | ||
+ | </ | ||
+ | < | ||
+ | <p>LH INS for insulin; LH VITC for vitamin C analysis</ | ||
+ | </ | ||
+ | <td > | ||
+ | <p>LH B1, LH C1, LH C2, LH C3 </ | ||
+ | </ | ||
+ | </tr> | ||
+ | <tr > | ||
+ | <td > | ||
+ | < | ||
+ | </td> | ||
+ | <td > | ||
+ | <p>1 x 85ml CPDA (for cell lines if required) (CP N1)</ | ||
+ | </td> | ||
+ | <td> | ||
+ | < | ||
+ | </td> | ||
+ | <td > | ||
+ | < | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr > | ||
+ | <td > | ||
+ | < | ||
+ | </td> | ||
+ | <td > | ||
+ | <p>1 x 7.5ml Serum tube (SE N1)</ | ||
+ | </td> | ||
+ | <td> | ||
+ | <p> Samples from the two serum tubes were used to produce priority aliquots SE A1 for TFTs and SE A2 for analyses at NHR</ | ||
+ | </td> | ||
+ | <td > | ||
+ | <p>SE B1, SE C1, SE C2</ | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr > | ||
+ | <td > | ||
+ | < | ||
+ | </td> | ||
+ | <td > | ||
+ | <p>1 x 7.5ml Serum tube (SE N2)</ | ||
+ | </td> | ||
+ | <td> | ||
+ | <p> Samples from the two serum tubes were used to produce priority aliquots SE A1 for TFTs and SE A2 for analyses at NHR</ | ||
+ | </td> | ||
+ | <td > | ||
+ | <p>SE B1, SE C1, SE C2</ | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr > | ||
+ | <td > | ||
+ | < | ||
+ | </td> | ||
+ | <td > | ||
+ | <p>1 x 4.9ml EDTA tube (E N3)</ | ||
+ | </td> | ||
+ | <td> | ||
+ | < | ||
+ | </td> | ||
+ | <td > | ||
+ | <p>E B1, E C1</ | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr > | ||
+ | <td > | ||
+ | < | ||
+ | </td> | ||
+ | <td > | ||
+ | <p>1 x 10ml Citrate tube (CI N1)</ | ||
+ | </td> | ||
+ | <td> | ||
+ | < | ||
+ | </td> | ||
+ | <td > | ||
+ | <p>CI B1, CI C1, CI C2</ | ||
+ | </td> | ||
+ | </tr> | ||
+ | </ | ||
+ | </ | ||
+ | </ | ||
+ | *Blood tubes/ | ||
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+ | |||
+ | \\ \\ | ||
+ | __Urine__ | ||
+ | |||
+ | Overnight urine was collected, however the protocol for processing and storing the samples was changed during the data collection, resulting in a different number of aliquots being collected compared to the later period. The original protocol was inappropriate for albumin measurement as they should have been centrifuged; | ||
+ | \\ \\ | ||
+ | __Saliva__ | ||
+ | |||
+ | The collection of saliva samples only began after a pilot study, and the protocol consisted of one sample collected on the day of the clinical assessment and a further three at home. HNR received all of these first samples, but for about 68 people (3%) no further samples were received. The collected saliva samples were sent to Germany for cortisol analysis before being returned to HNR. | ||
+ | |||
+ | ---- | ||
+ | \\ | ||
+ | === Age 68-70=== | ||
+ | |||
+ | At 68-70 years non-fasting blood samples were collected at a home visit by research nurses. | ||
+ | |||
+ | A number of assays have been undertaken and the remaining aliquots of EDTA, citrate plasma and serum are stored at the Bristol Bioresource Laboratories. | ||
+ | {{mrepo: | ||
+ | ---- | ||
+ | \\ | ||
+ | ^ | ||
- | ^ Table 2 - Biomarkers available at age 53 and 63. Biomarkers highlighted in bold are not present in other UK birth cohorts | + | ^System |
- | ^System | + | |**Metabolic** |
- | |**Metabolic** | + | | ::: |
- | | ::: | + | | ::: |
- | | ::: | + | | ::: |Cholesterol, |
- | | ::: |Triglycerides | + | | ::: |Triglycerides |
- | | ::: |Glycated Haemoglobin, | + | | ::: |
- | | ::: | + | |
| ::: | | ::: | ||
| ::: | | ::: | ||
| ::: | | ::: | ||
| ::: | | ::: | ||
- | | ::: | + | | ::: |Glycated Haemoglobin, |
|**Diet & Iron Markers**| | |**Diet & Iron Markers**| | ||
- | | ::: | + | | ::: |
| ::: | | ::: | ||
| ::: | | ::: | ||
| ::: | | ::: | ||
|**Inflammatory** | |**Inflammatory** | ||
- | | ::: | + | | ::: |
| ::: | | ::: | ||
- | | ::: | + | | ::: |
| ::: | | ::: | ||
| ::: | | ::: | ||
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| ::: | | ::: | ||
| ::: | | ::: | ||
- | | ::: | + | | ::: |
| ::: | | ::: | ||
| ::: | | ::: | ||
| ::: | | ::: | ||
- | | ::: | + | | ::: |
- | | ::: | + | | ::: |
- | | ::: | + | | ::: |
| ::: | | ::: | ||
| ::: | | ::: | ||
| ::: | | ::: | ||
- | | ::: | + | | ::: |
- | | ::: | + | | ::: |
- | |**Neuro-Endocrine** | + | |**Neuro-Endocrine** |
- | | ::: |Insulin Growth Factor 1, IGF-1 (nmol/L) |DHEAS (umol/ | + | | ::: |DHEAS (umol/L) |DHEAS (umol/ |
- | | ::: |Sex hormone binding globulin, SHBG (nmol/ | + | | ::: |Insulin Growth Factor 1, IGF-1 (nmol/ |
- | | ::: |Testosterone (nmol/ | + | | ::: |
| ::: | | ::: | ||
- | | ::: | + | | ::: |Sex hormone binding globulin, SHBG (nmol/ |
- | | ::: | + | | ::: |Testosterone (nmol/ |
| ::: | | ::: | ||
| ::: | | ::: | ||
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- | |||
- | ---- | ||
- | |||
- | === DNA === | ||
- | |||
- | Around 2,900 study members have given consent for DNA to be extracted | ||
- | from blood and / or buccal samples and LHA was given ethical approval in | ||
- | 2007 to use these samples for genetic studies of lifelong health and | ||
- | ageing. \\ | ||
- | Genetic studies help us to elucidate biological mechanisms of | ||
- | ageing and understand why people with similar life experiences age | ||
- | differently. | ||
- | |||
- | The NSHD DNA Repository supports high calibre studies of human health | ||
- | and disease that make the best use of the genetic resource in | ||
- | conjunction with the existing life course data. Dr Andrew Wong | ||
- | coordinates the genetic research of the NSHD and is responsible for | ||
- | the safe storage and efficient use of the samples, which are managed | ||
- | by Source BioScience. | ||
- | |||
- | {{mrepo: | ||
- | available. | ||