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mrepo:topics:biologicalsamples [16/05/2018 @ 11:05]
adammoore
mrepo:topics:biologicalsamples [03/10/2018 @ 16:42]
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 Stored biological samples available from [[questionnaires:​1999|1999]] comprise of EDTA plasma, Stored biological samples available from [[questionnaires:​1999|1999]] comprise of EDTA plasma,
 lithium heparin plasma, cell lines, and DNA.  The following tables list the current data available from these samples.\\ \\  lithium heparin plasma, cell lines, and DNA.  The following tables list the current data available from these samples.\\ \\ 
-{{mrepo:bloods_1999.jpg?​600|bloods collected in 1999}}+<​HTML>​ 
 +<table x:str=""​ style="​border-collapse:​ collapse; background-color:​ rgb(255, 255, 255); width: 900px;"​ border="​2"​ cellpadding="​2"​ cellspacing="​2">​ 
 +<​tbody>​ 
 +<tr style="​height:​ 12.75pt;" height="​17">​ 
 +<td class="​xl22"​ style="​text-align:​ center; width: 100px;"​ x:num> 
 +<​p><​b>​Order of Collection</​b></​p>​ 
 +</​td>​ 
 +<td class="​xl22"​ style="​text-align:​ center; width: 100px;"​ x:num> 
 +<​p><​b>​Blood Tube</​b></​p>​ 
 +</​td>​ 
 +<td class="​xl22"​ style="​text-align:​ center; width: 300px;"​ x:num> 
 +<​p><​b>​Analytes (destination)</​b></​p>​ 
 +</​td>​ 
 +<td class="​xl22"​ style="​text-align:​ center; width: 200px;"​ x:num> 
 +<​p><​b>​Repository aliquots</​b></​p>​ 
 +</​td>​ 
 +<td class="​xl22"​ style="​text-align:​ center; width: 200px;"​ x:num> 
 +<​p><​b>​Comment</​b></​p>​ 
 +</​td>​ 
 +</​tr>​ 
 +<​tr>​ 
 +<td > 
 +<​p>​1</​p>​ 
 +</​td>​ 
 +<td > 
 +<​p>​Buccal swabs</​p>​ 
 +</​td>​ 
 +<​td>​ 
 +<​p>​DNA (MRC-HBGU)</​p>​ 
 +</​td>​ 
 +<td > 
 +<​p>​DNA</​p>​ 
 +</​td>​ 
 +<td > 
 +<​p>​DNA quantified by Genprobe</​p>​ 
 +</​td>​ 
 +</​tr>​ 
 + 
 +<​tr>​ 
 +<td > 
 +<​p>​2</​p>​ 
 +</​td>​ 
 +<td > 
 +<p>1 x 3ml EDTA tube</​p>​ 
 +</​td>​ 
 +<​td>​ 
 +<​p>​DNA (MRC-HBGU)</​p>​ 
 +</​td>​ 
 +<td > 
 +<​p>​DNA</​p>​ 
 +</​td>​ 
 +<td > 
 +<​p>​Limited availability</​p>​ 
 +</​td>​ 
 +</​tr>​ 
 + 
 +<​tr>​ 
 +<td > 
 +<​p>​3</​p>​ 
 +</​td>​ 
 +<td > 
 +<p>1 x 2ml EDTA tube</​p>​ 
 +</​td>​ 
 +<​td>​ 
 +<​p>​DNA (MRC-HBGU)</​p>​ 
 +</​td>​ 
 +<td > 
 +<​p>​DNA</​p>​ 
 +</​td>​ 
 +<td > 
 +<​p>​Limited availability</​p>​ 
 +</​td>​ 
 +</​tr>​ 
 + 
 +<​tr>​ 
 +<td > 
 +<​p>​4</​p>​ 
 +</​td>​ 
 +<td > 
 +<p>1 x 6ml ACD tube</​p>​ 
 +</​td>​ 
 +<​td>​ 
 +<​p>​ECACC</​p>​ 
 +</​td>​ 
 +<td > 
 +<p> ECACC </​p>​ 
 +</​td>​ 
 +<td > 
 +<​p>​EBV transformed lymphoid cell lines created</​p>​ 
 +</​td>​ 
 +</​tr>​ 
 + 
 +<tr > 
 +<td > 
 +<​p>​5</​p>​ 
 +</​td>​ 
 +<td > 
 +<p>1 x 4ml Lithium heparin tube</​p>​ 
 +</​td>​ 
 +<​td>​ 
 +<​p>​Cholesterol,​ HDL cholesterol,​ triglyceride (RVI) </​p>​ 
 +</​td>​ 
 +<td > 
 +<​p>​Lithium heparin </​p>​ 
 +</​td>​ 
 +<td > 
 +<​p>​Limited availability </​p>​ 
 +</​td>​ 
 +</​tr>​ 
 + 
 +<tr > 
 +<td > 
 +<​p>​6</​p>​ 
 +</​td>​ 
 +<td > 
 +<p>1 x 3ml EDTA tube</​p>​ 
 +</​td>​ 
 +<​td>​ 
 +<​p>​Red cell folate, plasma folate, plasma B12, plasma ferritin (RVI)</​p>​ 
 +</​td>​ 
 +<td > 
 +<​p>​EDTA plasma</​p>​ 
 +</​td>​ 
 +<td > 
 +<​p>​Limited availability </​p>​ 
 +</​td>​ 
 +</​tr>​ 
 +</​tbody>​ 
 +</​table>​ 
 +</​HTML>​ 
 + 
 + 
 + 
 + 
 ---- ----
 \\  \\ 
 === Age 60-64=== === Age 60-64===
-__==Blood==__+__Blood__
  
- +At 60-64 years, fasting blood, saliva and overnight urine samples were collected, either in Clinical Research Facilities or at home. The volume of blood and processing protocol was designed to provide aliquots suitable for the analysis of biomarkers identified by LHA and its collaborators to be relevant to ageing and health. The collection and storage of the biological samples was organised by MRC Human Nutrition Research (HNR) in Cambridge.  ​
-At 60-64 years, fasting blood, saliva and overnight urine samples were collected, either in Clinical Research Facilities or at home. The volume of blood and processing protocol was designed to provide aliquots suitable for the analysis of biomarkers identified by LHA and its collaborators to be relevant to ageing and health.  ​+
  
 Up to 55ml of fasting blood was obtained from the study members, with a smaller volume being collected at remote home visits because the time to processing was too long to maintain the integrity of some analytes. ​ For example, potassium, creatinine and phosphate results were excluded from the reports of samples collected during remote home visits as the overnight delay before separation meant the result would not reflect the in vivo concentrations of these analytes. ​ Up to 55ml of fasting blood was obtained from the study members, with a smaller volume being collected at remote home visits because the time to processing was too long to maintain the integrity of some analytes. ​ For example, potassium, creatinine and phosphate results were excluded from the reports of samples collected during remote home visits as the overnight delay before separation meant the result would not reflect the in vivo concentrations of these analytes. ​
  
 DNA extractions were conducted on the EDTA tube used in the first round blood haematology analysis. Samples for lymphoid cell line transformations were originally collected in 1999; for those that did not previously provide a sample, one was collected in 2006-10. \\ \\  DNA extractions were conducted on the EDTA tube used in the first round blood haematology analysis. Samples for lymphoid cell line transformations were originally collected in 1999; for those that did not previously provide a sample, one was collected in 2006-10. \\ \\ 
-{{mrepo:bloods_0610.jpg?​600|bloods ​collected in 2006-10}}+ 
 +<​HTML>​ 
 +<table x:str=""​ style="​border-collapse:​ collapse; background-color:​ rgb(255, 255, 255); width: 900px;"​ border="​2"​ cellpadding="​2"​ cellspacing="​2">​ 
 +<​tbody>​ 
 +<tr style="​height:​ 12.75pt;" height="​17">​ 
 +<td class="​xl22"​ style="​text-align:​ center; width: 100px;"​ x:num> 
 +<​p><​b>​Order of collection priority</​b></​p>​ 
 +</​td>​ 
 +<td class="​xl22"​ style="​text-align:​ center; width: 300px;"​ x:num> 
 +<​p><​b>​Blood Tube</​b></​p>​ 
 +</​td>​ 
 +<td class="​xl22"​ style="​text-align:​ center; width: 300px;"​ x:num> 
 +<​p><​b>​Aliquots for first round blood analysis</​b></​p>​ 
 +</​td>​ 
 +<td class="​xl22"​ style="​text-align:​ center; width: 200px;"​ x:num> 
 +<​p><​b>​Repository aliquots</​b></​p>​ 
 +</​td>​ 
 +</​tr>​ 
 +<​tr>​ 
 +<td > 
 +<​p>​1</​p>​ 
 +</​td>​ 
 +<td > 
 +<p>1 x 2.7ml EDTA tube (EN1)</​p>​ 
 +</​td>​ 
 +<​td>​ 
 +<​p>​None as sample was sent for haematology analysis</​p>​ 
 +</​td>​ 
 +<td > 
 +<​p>​EN1 generated for DNA extraction</​p>​ 
 +</​td>​ 
 +</​tr>​ 
 + 
 +<​tr>​ 
 +<td > 
 +<​p>​2</​p>​ 
 +</​td>​ 
 +<td > 
 +<p>1 x 2.7ml EDTA tube (EN2)</​p>​ 
 +</​td>​ 
 +<​td>​ 
 +<p> None as sample was sent for HbA1c and red cell folate analysis <p> 
 +</​td>​ 
 +<td > 
 +<​p>​None</​p>​ 
 +</​td>​ 
 +</​tr>​ 
 + 
 +<​tr>​ 
 +<td > 
 +<​p>​3</​p>​ 
 +</​td>​ 
 +<td > 
 +<p>1 x 2.7ml Fluoride tube (FL N1)</​p>​ 
 +</​td>​ 
 +<​td>​ 
 +<​p>​FLA1 for glucose analysis</​p>​ 
 +</​td>​ 
 +<td > 
 +<​p>​None</​p>​ 
 +</​td>​ 
 +</​tr>​ 
 + 
 +<​tr>​ 
 +<td > 
 +<​p>​4*</​p>​ 
 +</​td>​ 
 +<td > 
 +<p>1 x 7.5ml Lithium heparin tube (trace metal) (LH N1)</​p>​ 
 +</​td>​ 
 +<​td>​ 
 +<p>LH INS for insulin; LH VITC for vitamin C analysis</​p>​ 
 +</​td>​ 
 +<td > 
 +<p>LH B1, LH C1, LH C2, LH C3 </​p>​ 
 +</​td>​ 
 +</​tr>​ 
 + 
 +<tr > 
 +<td > 
 +<​p>​5</​p>​ 
 +</​td>​ 
 +<td > 
 +<p>1 x 8.5ml CPDA (for cell lines if required) (CP N1)</​p>​ 
 +</​td>​ 
 +<​td>​ 
 +<​p>​None as sent to ECACC for cell lines</​p>​ 
 +</​td>​ 
 +<td > 
 +<​p>​None </​p>​ 
 +</​td>​ 
 +</​tr>​ 
 + 
 +<tr > 
 +<td > 
 +<​p>​6</​p>​ 
 +</​td>​ 
 +<td > 
 +<p>1 x 7.5ml Serum tube (SE N1)</​p>​ 
 +</​td>​ 
 +<​td>​ 
 +<p> Samples from the two serum tubes were used to produce priority aliquots SE A1 for TFTs and SE A2 for analyses at NHR</​p>​ 
 +</​td>​ 
 +<td > 
 +<p>SE B1, SE C1, SE C2</​p>​ 
 +</​td>​ 
 +</​tr>​ 
 + 
 +<tr > 
 +<td > 
 +<​p>​7*</​p>​ 
 +</​td>​ 
 +<td > 
 +<p>1 x 7.5ml Serum tube (SE N2)</​p>​ 
 +</​td>​ 
 +<​td>​ 
 +<p> Samples from the two serum tubes were used to produce priority aliquots SE A1 for TFTs and SE A2 for analyses at NHR</​p>​ 
 +</​td>​ 
 +<td > 
 +<p>SE B1, SE C1, SE C2</​p>​ 
 +</​td>​ 
 +</​tr>​ 
 + 
 +<tr > 
 +<td > 
 +<​p>​8*</​p>​ 
 +</​td>​ 
 +<td > 
 +<p>1 x 4.9ml EDTA tube (E N3)</​p>​ 
 +</​td>​ 
 +<​td>​ 
 +<​p>​None</​p>​ 
 +</​td>​ 
 +<td > 
 +<p>E B1, E C1</​p>​ 
 +</​td>​ 
 +</​tr>​ 
 +<tr > 
 +<td > 
 +<​p>​8*</​p>​ 
 +</​td>​ 
 +<td > 
 +<p>1 x 10ml Citrate tube (CI N1)</​p>​ 
 +</​td>​ 
 +<​td>​ 
 +<​p>​None</​p>​ 
 +</​td>​ 
 +<td > 
 +<p>CI B1, CI C1, CI C2</​p>​ 
 +</​td>​ 
 +</​tr>​ 
 + 
 +</​tbody>​ 
 +</​table>​ 
 +</​HTML>​ 
 +*Blood tubes/​aliquots not collected ​at remote home visits 
 + 
 + 
 + 
 + 
 + 
 + 
 +\\ \\  
 +__Urine__ 
 + 
 +Overnight urine was collected, however the protocol for processing and storing the samples was changed during the data collection, resulting ​in a different number of aliquots being collected compared to the later period. The original protocol was inappropriate for albumin measurement as they should have been centrifuged;​ and they were stored at -20ºC instead of -80ºC. The change in protocol resulted in an additional aliquot of urine being centrifuged and stored at -80ºC. The date of collection indicated whether the revised protocol was used or not.  
 +\\ \\  
 +__Saliva__ 
 + 
 +The collection of saliva samples only began after a pilot study, and the protocol consisted of one sample collected on the day of the clinical assessment and a further three at home. HNR received all of these first samples, but for about 68 people (3%) no further samples were received. The collected saliva samples were sent to Germany for cortisol analysis before being returned to HNR. 
 ---- ----
 \\  \\ 
 === Age 68-70=== === Age 68-70===
  
-\\ +At 68-70 years non-fasting blood samples were collected at a home visit by research nurses. ​ Prior to posting off for analysis, serum samples were allowed to clot for 15 minutes before being spun.  A number of biomarkers were measured: haemoglobin,​ HbA1C, total-, LDL-, HDL- cholesterol,​ triglycerides.  
 +  
 +A number of assays have been undertaken and the remaining aliquots of EDTA, citrate plasma and serum are stored at the Bristol Bioresource Laboratories. ​ DNA extractions have been conducted at MRC Epidemiology,​ Cambridge, as per the 2006-10 samples. ​ \\ \\ 
  
 +<​HTML>​
 +<table x:​str=""​ style="​border-collapse:​ collapse; background-color:​ rgb(255, 255, 255); width: 900px;"​ border="​2"​ cellpadding="​2"​ cellspacing="​2">​
 +<​tbody>​
 +<tr style="​height:​ 12.75pt;"​ height="​17">​
 +<td class="​xl22"​ style="​text-align:​ center; width: 100px;"​ x:num>
 +<​p><​b>​Order of collection priority</​b></​p>​
 +</td>
 +<td class="​xl22"​ style="​text-align:​ center; width: 300px;"​ x:num>
 +<​p><​b>​Blood Tube</​b></​p>​
 +</td>
 +<td class="​xl22"​ style="​text-align:​ center; width: 300px;"​ x:num>
 +<​p><​b>​Aliquots for first round blood analysis</​b></​p>​
 +</td>
 +<td class="​xl22"​ style="​text-align:​ center; width: 200px;"​ x:num>
 +<​p><​b>​Repository aliquots</​b></​p>​
 +</td>
 +</tr>
 +<tr>
 +<td >
 +<​p>​1</​p>​
 +</td>
 +<td >
 +<p>1 x 2.7ml citrate</​p>​
 +</td>
 +<td>
 +<​p>​None </p>
 +</td>
 +<td >
 +<​p>​CIT101 – CIT104</​p>​
 +</td>
 +</tr>
  
 +<tr>
 +<td >
 +<​p>​2</​p>​
 +</td>
 +<td >
 +<p>1 x 5ml Serum</​p>​
 +</td>
 +<td>
 +<p> Cholesterol,​ HDL-, LDL-cholesterol,​ triglycerides<​p>​
 +</td>
 +<td >
 +<​p>​SEA101 – SEA107</​p>​
 +</td>
 +</tr>
  
 +<tr>
 +<td >
 +<​p>​3</​p>​
 +</td>
 +<td >
 +<p>1 x 5ml Serum</​p>​
 +</td>
 +<td>
 +<​p>​None</​p>​
 +</td>
 +<td >
 +<​p>​SEA201 – SEA207</​p>​
 +</td>
 +</tr>
  
 +<tr>
 +<td >
 +<​p>​4</​p>​
 +</td>
 +<td >
 +<p>1 x 5ml Serum</​p>​
 +</td>
 +<td>
 +<​p>​None</​p>​
 +</td>
 +<td >
 +<​p>​SEA301 – SEA307 </p>
 +</td>
 +</tr>
  
 +<tr >
 +<td >
 +<​p>​5</​p>​
 +</td>
 +<td >
 +<p>1 x 4ml EDTA</​p>​
 +</td>
 +<td>
 +<​p>​HbA1c,​ haemoglobin,​ FBC, WCC</​p>​
 +</td>
 +<td >
 +<​p>​DNA </p>
 +</td>
 +</tr>
  
 +<tr >
 +<td >
 +<​p>​6</​p>​
 +</td>
 +<td >
 +<p>1 x 4ml EDTA </p>
 +</td>
 +<td>
 +<p> None</​p>​
 +</td>
 +<td >
 +<​p>​EN2_01 – EN2_05</​p>​
 +</td>
 +</tr>
  
 +</​tbody>​
 +</​table>​
 +</​HTML>​
  
  
  
 +----
 +\\ 
  
  
  
 +^       ​Biomarkers available since age 53. Biomarkers highlighted in bold are not present in other UK birth cohorts (as far as is known) ​ ^^^^
  
-^       Table 2  - Biomarkers available at age 53 and 63. Biomarkers highlighted in bold are not present in other UK birth cohorts ​  ^^^ +^System ​            ​^Biomarkers at 53 years                               ^Biomarkers at 60-64 years                               ​^Biomarkers at 68-70 years                        ​
-^System ​            ​^Biomarkers at 53 yrs                               ^Biomarkers at 63 yrs                               ^ +|**Metabolic** ​         |                     ​|Adiponectin (μg/​mL) ​                               ​|
-|**Metabolic** ​         |Cholesterol,​ Total (mmol/​L) ​                       ​|Adiponectin (μg/​mL) ​                               | +| :::               ​|Cholesterol, ​Total (mmol/​L) ​                   |CholesterolTotal (mmol/​L) ​                       |Cholesterol,​ Total (mmol/​L) ​                       | 
-| :::               ​|Cholesterol, ​High density LipoproteinHDL (mmol/​L)|Cholesterol,​ Total (mmol/​L) ​                       | +| :::               ​|Cholesterol, ​High density Lipoprotein,​ LDL (mmol/L) |Cholesterol,​ High density Lipoprotein,​ HDL (mmol/​L)|Cholesterol,​ High density Lipoprotein,​ HDL (mmol/L)| 
-| :::               ​|Cholesterol, ​Low density Lipoprotein,​ LDL (mmol/L) |Cholesterol,​ High density Lipoprotein,​ HDL (mmol/L)| +| :::               |Cholesterol,​ Low density Lipoprotein,​ LDL (mmol/L) |Cholesterol,​ Low density Lipoprotein,​ LDL (mmol/L) |Cholesterol,​ Low density Lipoprotein,​ LDL (mmol/L) | 
-| :::               |Triglycerides ​(mmol/​L) ​                            ​|Cholesterol,​ Low density Lipoprotein,​ LDL (mmol/L) | +| :::               |Triglycerides ​(mmol/L                            ​|Triglycerides (mmol/​L) ​                            |Triglycerides (mmol/​L) ​  ​| ​                         
-| :::               |Glycated Haemoglobin,​ HbA1c (mmol/mol            ​|Triglycerides (mmol/​L) ​                            | +| :::               ​| ​                                                  ​|Glucose (nmol/​L) ​                                  ​| ​
-| :::               ​| ​                                                  ​|Glucose (nmol/​L) ​                                  |+
 | :::               ​| ​                                                  ​|Insulin (pmol/​L) ​                                  | | :::               ​| ​                                                  ​|Insulin (pmol/​L) ​                                  |
 | :::               ​| ​                                                  ​|Leptin (ng/​mL) ​                                    | | :::               ​| ​                                                  ​|Leptin (ng/​mL) ​                                    |
 | :::               ​| ​                                                  ​|Lipoprotein[a] (mg/​dL) ​                            | | :::               ​| ​                                                  ​|Lipoprotein[a] (mg/​dL) ​                            |
 | :::               ​| ​                                                  ​|Proinsulin (pmol/​L) ​                               | | :::               ​| ​                                                  ​|Proinsulin (pmol/​L) ​                               |
-| :::               ​| ​                                                  ​|Glycated Haemoglobin,​ HbA1c (mmol/​mol) ​            |+| :::               |Glycated Haemoglobin,​ HbA1c (mmol/​mol) ​            |Glycated Haemoglobin,​ HbA1c (mmol/​mol) ​            |Glycated Haemoglobin,​ HbA1c (mmol/​mol) ​           ​|
 |**Diet & Iron Markers**| ​                                                  ​|Ferritin (ng/​mL) ​                                  | |**Diet & Iron Markers**| ​                                                  ​|Ferritin (ng/​mL) ​                                  |
-| :::               ​| ​                                                  ​|Haemoglobin,​ Hb (g/L)                              |+| :::               ​| ​                                                  |Haemoglobin,​ Hb (g/L)                              ​|Haemoglobin,​ Hb (g/L)                              |
 | :::               ​| ​                                                  |** Iron (μg/dL) **                                 | | :::               ​| ​                                                  |** Iron (μg/dL) **                                 |
 | :::               ​| ​                                                  ​|Vitamin C (μmol/​L) ​                                | | :::               ​| ​                                                  ​|Vitamin C (μmol/​L) ​                                |
 | :::               ​| ​                                                  ​|Vitamin D3 (250HD3) (ng/​mL)† ​                      | | :::               ​| ​                                                  ​|Vitamin D3 (250HD3) (ng/​mL)† ​                      |
 |**Inflammatory** ​      ​| ​                                                  |** Anti-Thyroperoxidase,​ TPO (IU/mL) **            | |**Inflammatory** ​      ​| ​                                                  |** Anti-Thyroperoxidase,​ TPO (IU/mL) **            |
-| :::               ​| ​                                                  ​|Basophils (x109/L)                                 |+| :::               ​| ​                                                  ​|Basophils (x10<​sup>​9</​sup>/​L) ​                                ​|Basophils (x10<​sup>​9</​sup>​/L)                                 |
 | :::               ​| ​                                                  ​|C-Reactive Protein, CRP (mg/​L) ​                    | | :::               ​| ​                                                  ​|C-Reactive Protein, CRP (mg/​L) ​                    |
-| :::               ​| ​                                                  |Eosinophil ​(x109/L)                                |+| :::               ​| ​                                                  |Eosinophils ​(x10<​sup>​9</​sup>/​L) ​                              ​|Eosinophils (x10<​sup>​9</​sup>​/L)                                |
 | :::               ​| ​                                                  ​|Fibrinogen (g/L)                                   | | :::               ​| ​                                                  ​|Fibrinogen (g/L)                                   |
 | :::               ​| ​                                                  |** Homocysteine concentration (μmol/L) **          | | :::               ​| ​                                                  |** Homocysteine concentration (μmol/L) **          |
Line 77: Line 488:
 | :::               ​| ​                                                  |Von Willebrand Factor, VWF (IU/​dL) ​                | | :::               ​| ​                                                  |Von Willebrand Factor, VWF (IU/​dL) ​                |
 | :::               ​| ​                                                  |Red Cell Folate, RCF (nmol/​L) ​                     | | :::               ​| ​                                                  |Red Cell Folate, RCF (nmol/​L) ​                     |
-| :::               ​| ​                                                  ​|Lymphocytes (x109/L)                               |+| :::               ​| ​                                                  ​|Lymphocytes (x10<​sup>​9</​sup>/​L) ​                              ​|Lymphocytes (x10<​sup>​9</​sup>​/L)                               |
 | :::               ​| ​                                                  |Mean Corpuscular Volume, MCV                       | | :::               ​| ​                                                  |Mean Corpuscular Volume, MCV                       |
 | :::               ​| ​                                                  |Mean Corpuscular Haemoglobin,​ MCH                  | | :::               ​| ​                                                  |Mean Corpuscular Haemoglobin,​ MCH                  |
 | :::               ​| ​                                                  |** Mean Platelet Volume, MPV **                    | | :::               ​| ​                                                  |** Mean Platelet Volume, MPV **                    |
-| :::               ​| ​                                                  ​|Monocytes (x109/L)                                 | +| :::               ​| ​                                                  ​|Monocytes (x10<​sup>​9</​sup>/​L) ​                                ​|Monocytes (x10<​sup>​9</​sup>​/L)                                 | 
-| :::               ​| ​                                                  ​|Neutrophils (x109/L)                               | +| :::               ​| ​                                                  ​|Neutrophils (x10<​sup>​9</​sup>/​L) ​                              ​|Neutrophils (x10<​sup>​9</​sup>​/L)                               | 
-| :::               ​| ​                                                  ​|Platelet count (x109/L)                            |+| :::               ​| ​                                                  ​|Platelet count (x10<​sup>​9</​sup>/​L) ​                           |Platelet count (x10<​sup>​9</​sup>​/L)                            |
 | :::               ​| ​                                                  |** Platelet Distribution Width, PDW (%) **         | | :::               ​| ​                                                  |** Platelet Distribution Width, PDW (%) **         |
 | :::               ​| ​                                                  |** Potassium (mmol/L) **                           | | :::               ​| ​                                                  |** Potassium (mmol/L) **                           |
 | :::               ​| ​                                                  ​|Protein (blood), Total (g/L)                       | | :::               ​| ​                                                  ​|Protein (blood), Total (g/L)                       |
-| :::               ​| ​                                                  |Red Blood Cell, RBC, count (109/L)                 ​+| :::               ​| ​                                                  |Red Blood Cell, RBC, count (x10<​sup>​12</​sup>​/L)                   |Red Blood Cell, RBC, count (x10<​sup>​12</​sup>/​L) ​                  
-| :::               ​| ​                                                  ​|White Blood Cell, WBC, count (109/L)               ​+| :::               ​| ​                                                  ​|White Blood Cell, WBC, count (x10<​sup>​9</​sup>​/L)                 |White Blood Cell, WBC, count (x10<​sup>​9</​sup>/​L) ​                
-|**Neuro-Endocrine** ​   |DHEAS (umol/​L) ​                                    |Cortisol (saliva) (nmol/​L) ​                        | +|**Neuro-Endocrine** ​   |                                     ​|Cortisol (saliva) (nmol/​L) ​                        | 
-| :::               |Insulin Growth Factor 1, IGF-1 (nmol/L)            |DHEAS (umol/​L) ​                                    | +| :::               |DHEAS (umol/L)            |DHEAS (umol/​L) ​                                    | 
-| :::               |Sex hormone binding globulinSHBG (nmol/​L) ​       |Insulin Growth Factor 1, IGF-1 (nmol/​L) ​           +| :::               |Insulin Growth Factor 1IGF-1 (nmol/​L) ​           |Insulin Growth Factor 1, IGF-1 (nmol/​L) ​       
-| :::               |Testosterone (nmol/​L) ​                             ​|Insulin Growth Factor 2, IGF-2 (nmol/​L) ​           |+| :::               ​| ​                             |Insulin Growth Factor 2, IGF-2 (nmol/​L) ​           |
 | :::               ​| ​                                                  ​|Insulin Growth Factor IGF-BP3 (ng/​mL) ​             | | :::               ​| ​                                                  ​|Insulin Growth Factor IGF-BP3 (ng/​mL) ​             |
-| :::               ​| ​                                                  |Sex hormone binding globulin, SHBG (nmol/​L) ​       | +| :::               |Sex hormone binding globulin, SHBG (nmol/​L) ​                                                  |Sex hormone binding globulin, SHBG (nmol/​L) ​       | 
-| :::               ​| ​                                                  ​|Testosterone (nmol/​L) ​                             |+| :::               |Testosterone (nmol/​L) ​                             ​|Testosterone (nmol/​L) ​                             |
 | :::               ​| ​                                                  ​|Thyroid tests (T3, T4) (pmol/​L) ​                   | | :::               ​| ​                                                  ​|Thyroid tests (T3, T4) (pmol/​L) ​                   |
 | :::               ​| ​                                                  |** Thyroid Stimulating Hormone, TSH (mU/L) **      | | :::               ​| ​                                                  |** Thyroid Stimulating Hormone, TSH (mU/L) **      |
Line 123: Line 534:
  
  
- 
-----  
- 
-=== DNA === 
- 
-Around 2,900 study members have given consent for DNA to be extracted 
-from blood and / or buccal samples and LHA was given ethical approval in 
-2007 to use these samples for genetic studies of lifelong health and 
-ageing. \\  
-Genetic studies help us to elucidate biological mechanisms of 
-ageing and understand why people with similar life experiences age 
-differently. 
- 
-The NSHD DNA Repository supports high calibre studies of human health 
-and disease that make the best use of the genetic resource in 
-conjunction with the existing life course data. Dr Andrew Wong 
-coordinates the genetic research of the NSHD and is responsible for 
-the safe storage and efficient use of the samples, which are managed 
-by Source BioScience. 
- 
-{{mrepo:​Guidance-NSHD_biological-samples.pdf|Further information}} on the access to the NSHD DNA Repository is 
-available.