==== Biological Samples ===== ---- === Age 53 === Biological samples were collected for the first time in [[questionnaires:1999|1999]]; non-fasting blood samples were used to measure indicators of risk factors for cardiovascular disease (cholesterol, HDL, LDL, triglyceride and glycoslated haemoglobin) and nutritional markers (red cell folate, plasma folate, plasma vitamin B12 and plasma ferritin). The samples also include a blood sample and a cheek swab from which DNA was derived. A further sample was taken and stored for future cell line cultures. Blood was taken using standard phlebotomy techniques using the BD vacutainer system. Stored biological samples available from [[questionnaires:1999|1999]] comprise of EDTA plasma, lithium heparin plasma, cell lines, and DNA. The following tables list the current data available from these samples.\\ \\
Order of Collection |
Blood Tube |
Analytes (destination) |
Repository aliquots |
Comment |
1 |
Buccal swabs |
DNA (MRC-HBGU) |
DNA |
DNA quantified by Genprobe |
2 |
1 x 3ml EDTA tube |
DNA (MRC-HBGU) |
DNA |
Limited availability |
3 |
1 x 2ml EDTA tube |
DNA (MRC-HBGU) |
DNA |
Limited availability |
4 |
1 x 6ml ACD tube |
ECACC |
ECACC |
EBV transformed lymphoid cell lines created |
5 |
1 x 4ml Lithium heparin tube |
Cholesterol, HDL cholesterol, triglyceride (RVI) |
Lithium heparin |
Limited availability |
6 |
1 x 3ml EDTA tube |
Red cell folate, plasma folate, plasma B12, plasma ferritin (RVI) |
EDTA plasma |
Limited availability |
Order of collection priority |
Blood Tube |
Aliquots for first round blood analysis |
Repository aliquots |
1 |
1 x 2.7ml EDTA tube (EN1) |
None as sample was sent for haematology analysis |
EN1 generated for DNA extraction |
2 |
1 x 2.7ml EDTA tube (EN2) |
None as sample was sent for HbA1c and red cell folate analysis
|
None |
3 |
1 x 2.7ml Fluoride tube (FL N1) |
FLA1 for glucose analysis |
None |
4* |
1 x 7.5ml Lithium heparin tube (trace metal) (LH N1) |
LH INS for insulin; LH VITC for vitamin C analysis |
LH B1, LH C1, LH C2, LH C3 |
5 |
1 x 8.5ml CPDA (for cell lines if required) (CP N1) |
None as sent to ECACC for cell lines |
None |
6 |
1 x 7.5ml Serum tube (SE N1) |
Samples from the two serum tubes were used to produce priority aliquots SE A1 for TFTs and SE A2 for analyses at NHR |
SE B1, SE C1, SE C2 |
7* |
1 x 7.5ml Serum tube (SE N2) |
Samples from the two serum tubes were used to produce priority aliquots SE A1 for TFTs and SE A2 for analyses at NHR |
SE B1, SE C1, SE C2 |
8* |
1 x 4.9ml EDTA tube (E N3) |
None |
E B1, E C1 |
8* |
1 x 10ml Citrate tube (CI N1) |
None |
CI B1, CI C1, CI C2 |
Order of collection priority |
Blood Tube |
Aliquots for first round blood analysis |
Repository aliquots |
1 |
1 x 2.7ml citrate |
None |
CIT101 – CIT104 |
2 |
1 x 5ml Serum |
Cholesterol, HDL-, LDL-cholesterol, triglycerides
|
SEA101 – SEA107 |
3 |
1 x 5ml Serum |
None |
SEA201 – SEA207 |
4 |
1 x 5ml Serum |
None |
SEA301 – SEA307 |
5 |
1 x 4ml EDTA |
HbA1c, haemoglobin, FBC, WCC |
DNA |
6 |
1 x 4ml EDTA |
None |
EN2_01 – EN2_05 |